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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene is observed in several tumors, which leads to chromosome instability, and therefore might contribute to tumorigenesis. This protein was uncovered to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial job in advertising and marketing cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are described. Two supplemental splice variants have been noted but in-depth nucleotide sequence data isn't nonetheless offered.

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Blocking peptides are peptides that bind precisely to your target antibody and block antibody binding. These peptide normally incorporates the epitope acknowledged via the antibody.

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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to be sure the absolute best results are...

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This guide outlines further doable optimization of mobile and immunoassay parameters to make sure the absolute best final results are...

Antibodies certain to the blocking peptide not bind for the epitope about the goal protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is unique.

Concentrate on Facts Cyclin E belongs on the hugely conserved cyclin loved ones, whose associates are characterised by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic party. Cyclin E varieties a fancy with and capabilities for a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S transition. This protein accumulates for the G1-S stage boundary and it is degraded as cells development by way of S period.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...

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